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1x pfu dna polymerase buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 1x pfu dna polymerase buffer
    1x Pfu Dna Polymerase Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+buffer/DNA/pmc12489705-42-12-32
    Average 99 stars, based on 1 article reviews
    1x pfu dna polymerase buffer - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Engineering color variants of green fluorescent protein (GFP) for thermostability, pH-sensitivity, and improved folding kinetics.
    Article Snippet: .. All TPCR mutagenesis reactions were performed in a final volume of 25 μl and included the following components: 13 ng of plasmid DNA pGFP (ASV), 20 nM of both forward and reverse primer, 200 μM of each dNTP, Pfu-Buffer (20 mM Tris–HCl (pH 8.8 at 25 °C), 10 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 1.0 % Triton X-100, and 1 mg/ml nuclease-free BSA), and 2.0 U of Pfu DNA polymerase (Fermentas). ..

    Plasmid Preparation:

    Article Title: Engineering color variants of green fluorescent protein (GFP) for thermostability, pH-sensitivity, and improved folding kinetics.
    Article Snippet: .. All TPCR mutagenesis reactions were performed in a final volume of 25 μl and included the following components: 13 ng of plasmid DNA pGFP (ASV), 20 nM of both forward and reverse primer, 200 μM of each dNTP, Pfu-Buffer (20 mM Tris–HCl (pH 8.8 at 25 °C), 10 mM KCl, 10 mM (NH4)2SO4, 2 mM MgSO4, 1.0 % Triton X-100, and 1 mg/ml nuclease-free BSA), and 2.0 U of Pfu DNA polymerase (Fermentas). ..

    Article Title: Virus-like particles of Macrobrachium rosenbergii nodavirus: Particle size and capsid protein assembly domain
    Article Snippet: Macrobrachium rosenbergii nodavirus (MrNv) causes white tail disease (WTD) in the giant freshwater prawn, which poses a major issue in the aquaculture sector.. The capsid protein (CP) of MrNv expressed in Escherichia coli self-assembles into virus-like particles (VLPs) with a triangulation number, T = 3.. However, the region in MrNv CP that involved in the viral capsid formation has yet to be identified.

    DNA Amplification:

    Article Title: Virus-like particles of Macrobrachium rosenbergii nodavirus: Particle size and capsid protein assembly domain
    Article Snippet: Macrobrachium rosenbergii nodavirus (MrNv) causes white tail disease (WTD) in the giant freshwater prawn, which poses a major issue in the aquaculture sector.. The capsid protein (CP) of MrNv expressed in Escherichia coli self-assembles into virus-like particles (VLPs) with a triangulation number, T = 3.. However, the region in MrNv CP that involved in the viral capsid formation has yet to be identified.

    Purification:

    Article Title: Virus-like particles of Macrobrachium rosenbergii nodavirus: Particle size and capsid protein assembly domain
    Article Snippet: Macrobrachium rosenbergii nodavirus (MrNv) causes white tail disease (WTD) in the giant freshwater prawn, which poses a major issue in the aquaculture sector.. The capsid protein (CP) of MrNv expressed in Escherichia coli self-assembles into virus-like particles (VLPs) with a triangulation number, T = 3.. However, the region in MrNv CP that involved in the viral capsid formation has yet to be identified.

    Polymerase Chain Reaction:

    Article Title: Pretreatment of lignocelluloses for enhanced biogas production: A review on influencing mechanisms and the importance of microbial diversity
    Article Snippet: .. The reaction mixture of both bacterial and archaeal PCR was prepared by addition of 0.5 of each primer, 1.5 mM MgCl2, 1 U of pfu polymerase enzyme and 1 × pfu buffer (Fermentas, Lithuania) and 0.2 mM dNTPs to 2 μL of the diluted DNA sample. ..

    Article Title: Analysis of acetohydroxyacid synthase1 gene in chickpea conferring resistance to imazamox herbicide.
    Article Snippet: Chickpea (Cicer arietinum L.) production in the Canadian prairies is challenging due to a lack of effective weed management mainly because of poor competition ability of the crop and limited registered herbicide options.. Chickpea genotype with resistance to imidazolinone (IMI) herbicides has been identified.. A point mutation in the acetohydroxyacid synthase1 (AHAS1) gene at C581 to T581, resulting in an amino acid substitution from Ala194 to Val194 (position 205, standardized to arabidopsis), confers the resistance to imazamox in chickpea.

    Amplification:

    Article Title: Analysis of acetohydroxyacid synthase1 gene in chickpea conferring resistance to imazamox herbicide.
    Article Snippet: Chickpea (Cicer arietinum L.) production in the Canadian prairies is challenging due to a lack of effective weed management mainly because of poor competition ability of the crop and limited registered herbicide options.. Chickpea genotype with resistance to imidazolinone (IMI) herbicides has been identified.. A point mutation in the acetohydroxyacid synthase1 (AHAS1) gene at C581 to T581, resulting in an amino acid substitution from Ala194 to Val194 (position 205, standardized to arabidopsis), confers the resistance to imazamox in chickpea.



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